Glutaminolysis and Fumarate Accumulation Integrate Immunometabolic and Epigenetic Programs in Trained Immunity
Cell Metab. 2016-12-13;24(6):807-819.
- Abstract
- Induction of trained immunity (innate immune memory) is mediated by activation of immune and metabolic pathways that result in epigenetic rewiring of cellular functional programs. Through network-level integration of transcriptomics and metabolomics data, we identify glycolysis, glutaminolysis, and the cholesterol synthesis pathway as indispensable for the induction of trained immunity by β-glucan in monocytes. Accumulation of fumarate, due to glutamine replenishment of the TCA cycle, integrates immune and metabolic circuits to induce monocyte epigenetic reprogramming by inhibiting KDM5 histone demethylases. Furthermore, fumarate itself induced an epigenetic program similar to β-glucan-induced trained immunity. In line with this, inhibition of glutaminolysis and cholesterol synthesis in mice reduced the induction of trained immunity by β-glucan. Identification of the metabolic pathways leading to induction of trained immunity contributes to our understanding of innate immune memory and opens new therapeutic avenues.
- Consortium data used in this publication
- The accession numbers for the RNA sequencing time course of control, b-glucan-trained, and LPS-immunotolerant monocytes and RNA sequencing and ChIP sequencing data of control and fumarate- and b-glucan-trained monocytes at day 6 reported in this paper are GEO: GSE85246 and GSE86940.
- Datasets
- DSR792SDR, DSR172GPZ, DSR393GUK, DSR361QLH, DSR165WZV, DSR031GBV, DSR291BUV, DSR466SLQ, DSR177MIJ, DSR797JQZ, DSR036KGB, DSR510KTT, DSR022LQE, DSR978NJL, DSR180XCR, DSR970XLO, DSR261HEQ, DSR626XPQ, DSR294TQM, DSR570QMF, DSR235FBB, DSR027MJY, DSR412HGU, DSR439QVH, DSR119GEO, DSR516MVY, DSR282UKE, DSR400QXY, DSR831GAV, DSR402OWE, DSR920OPS, DSR245WKV, DSR017REG, DSR104YYJ, DSR432IFA, DSR109EEK, DSR977WLQ, DSR397QCJ, DSR840QWJ, DSR826ZWP, DSR738OTN, DSR573CYC, DSR329ZII, DSR067KMF, DSR403KXI, DSR189NFY, DSR082KYM, DSR911UHJ, DSR108AZI, DSR016AJU, DSR781BAK, DSR860BBA, DSR356LED, DSR849WXC, DSR987DNE, DSR052GHS, DSR668PAN, DSR526KYA, DSR890NNI, DSR552EKW, DSR122CAJ, DSR909LYK, DSR851UZK, DSR777PED, DSR109ZXS, DSR455FHD, DSR062UBG, DSR070MUR, DSR767GNC, DSR704SCW, DSR217VBP, DSR793NBU, DSR471OUM, DSR903EQN, DSR358LMN, DSR654PMK, DSR382JSZ, DSR679XJR, DSR804JWH, DSR324UZU, DSR050XFQ, DSR297YTV, DSR010VSN, DSR160ZNS, DSR303WJG, DSR672IHC, DSR171SJU, DSR837PMC, DSR696DSB, DSR870QZR, DSR319IWK, DSR787ZVE, DSR074TRX, DSR789JAJ, DSR907EGY, DSR602CYK, DSR818FOJ, DSR040ZJJ, DSR859JQG, DSR871XCG, DSR833JIH, DSR186TDJ, DSR091JLV, DSR289VNA, DSR036RBB, DSR107AXO, DSR871PCP, DSR283IBC, DSR978KHJ, DSR197NWS, DSR978YES, DSR228VFG, DSR175OTX, DSR084XEY, DSR016TVA, DSR601REX, DSR080UMT, DSR438HAQ, DSR169TJA, DSR773FOT, DSR342UXO, DSR111VUO, DSR412YPY, DSR140ISE, DSR401DQT, DSR974CEF, DSR800ZKK, DSR935QGD, DSR080DNH, DSR261KXA, DSR229PCE, DSR796EAR, DSR650GPE, DSR877BRW, DSR821XCG, DSR541TCU, DSR112GDK, DSR577JTH, DSR546LIB, DSR397YCO, DSR258EDL, DSR999BSV, DSR026EDM, DSR245GGA, DSR468ZSR, DSR884JBU, DSR324LLG, DSR448SRG, DSR177BAQ, DSR348WZF, DSR582ZNR, DSR982TIR, DSR005KHX, DSR593NPV, DSR524WZB, DSR227LKV, DSR831PYL, DSR992NHC, DSR983LGA, DSR031EDT, DSR937QPF, DSR773CXD, DSR573VIF, DSR468OET, DSR324RXQ, DSR281QKE, DSR098PFJ, DSR857GDR, DSR030FPP, DSR244HDC, DSR110TAE, DSR383LEV, DSR716ZZT, DSR528PHX, DSR230NRH, DSR397ONR, DSR934UQW, DSR697HCX, DSR398SSK, DSR481SFJ, DSR121PJD, DSR046NHR, DSR659GJL, DSR855BYB, DSR186OAN, DSR742HYH, DSR713MBG, DSR249ZXB, DSR044QHH, DSR690ZQS, DSR874VEO, DSR387CHY, DSR861NMR, DSR908PCK, DSR894ESI, DSR040UNQ, DSR000FLL, DSR669BSO, DSR138VHU, DSR034ZNQ, DSR106KTE, DSR412GWK, DSR710EBY, DSR123SDA, DSR873SZS, DSR698TJB, DSR220BWN, DSR424JZN, DSR775NYE, DSR122IWH, DSR513ZPW, DSR588JIC, DSR772ERF, DSR324XXB, DSR126ZHG, DSR751JQK, DSR945INA, DSR252EWN, DSR541NWK, DSR594CIB, DSR806FTS, DSR877GMC, DSR277QAP, DSR358JPO, DSR451XYK, DSR331VTS, DSR571OEB, DSR843ENW, DSR778HGA, DSR136YMF, DSR049PCN, DSR634ADB, DSR920FDN, DSR875GGI, DSR642AXP, DSR472AWT, DSR110DSK, DSR260TIJ, DSR135OVL, DSR259WDX, DSR503VVK, DSR825AMW, DSR477MOZ, DSR298NAN, DSR856YOV, DSR901KOQ, DSR065OEH, DSR468YVV, DSR205EVJ, DSR660FOW, DSR215ALV, DSR751TNS, DSR102LRA, DSR968UJL, DSR359YWY, DSR703ZXV, DSR019CTT, DSR573QGD